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Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue.
Journal article

Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue.

  • Berezowska S Institute of Pathology, University of Bern, Murtenstrasse 31, 3010, Bern, Switzerland. sabina.berezowska@pathology.unibe.ch.
  • Galván JA Institute of Pathology, University of Bern, Murtenstrasse 31, 3010, Bern, Switzerland.
  • 2017-02-04
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2017
Autophagy
Immunohistochemistry
LC3
Sequestosome 1
p62/SQSTM1
Autophagy
Biomarkers
Humans
Immunohistochemistry
Microtubule-Associated Proteins
Neoplasms
Paraffin Embedding
Sequestosome-1 Protein
English Autophagy is a highly conserved cellular mechanism of "self digestion," ensuring cellular homeostasis, and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy.LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy, and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/157118
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