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Regeneration of cassava plants via shoot organogenesis.
Journal article

Regeneration of cassava plants via shoot organogenesis.

  • Li HQ Institute for Plant Sciences, Swiss Federal Institute of Technology, ETH Zentrum, CH-8092 Zürich, Switzerland Fax no.: +41-1-632-1044 E-mail: puonti-kaerlas@ipw.agrl.ethz.ch, , , , , , CH.
  • Guo JY South China Institute of Botany, Academia Sinica, Guagzhou, China, , , , , , CN.
  • Huang YW South China Institute of Botany, Academia Sinica, Guagzhou, China, , , , , , CN.
  • Liang CY South China Institute of Botany, Academia Sinica, Guagzhou, China, , , , , , CN.
  • Liu HX South China Institute of Botany, Academia Sinica, Guagzhou, China, , , , , , CN.
  • Potrykus I Institute for Plant Sciences, Swiss Federal Institute of Technology, ETH Zentrum, CH-8092 Zürich, Switzerland Fax no.: +41-1-632-1044 E-mail: puonti-kaerlas@ipw.agrl.ethz.ch, , , , , , CH.
  • Puonti-Kaerlas J Institute for Plant Sciences, Swiss Federal Institute of Technology, ETH Zentrum, CH-8092 Zürich, Switzerland Fax no.: +41-1-632-1044 E-mail: puonti-kaerlas@ipw.agrl.ethz.ch, , , , , , CH.
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  • 2019-02-10
Published in:
  • Plant cell reports. - 1998
Key words Cassava
Manihot esculenta Crantz
Organogenesis
Shoot regeneration
English A novel regeneration system based on direct shoot organogenesis is described for cassava. Plants could be regenerated at high frequency by inducing shoot primordia on explants derived from cotyledons of cassava somatic embryos. After a passage on elongation medium, the regenerated shoots were easily rooted in hormone-free medium and could be successfully transplanted to soil. Using the shoot-organogenesis-based regeneration method, up to eight transplantable plantlets per explant could be regenerated. The system was optimised first for one cassava cultivar, and then its transferability to three other cultivars was demonstrated. This method widens the scope of in vitro regeneration modes of cassava, and is also compatible with Agrobacterium-mediated transformation. To develop an efficient system for production of somatic embryos for regeneration experiments, conditions for inducing primary and cycling somatic embryos were also studied, and highly efficient plant regeneration via germination of somatic embryos was achieved using maltose instead of sucrose in the culture medium, and combining paclobutrazol with 2,4-dichlorophenoxyacetic acid in the embryo induction medium.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/158843
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