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Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives.

  • Baldi L Laboratory of Cellular Biotechnology, Ecole Polytechnique Fédérale de Lausanne, Station 6, 1015, Lausanne, Switzerland. Lucia.Baldi@epfl.ch
  • Hacker DL
  • Adam M
  • Wurm FM
  • 2007-01-20
Published in:
  • Biotechnology letters. - 2007
Animals
Biotechnology
Gene Expression
Gene Transfer Techniques
Genetic Vectors
Humans
Recombinant Proteins
English The expansion of the biologics pipeline depends on the identification of candidate proteins for clinical trials. Speed is one of the critical issues, and the rapid production of high quality, research-grade material for preclinical studies by transient gene expression (TGE) is addressing this factor in an impressive way: following DNA transfection, the production phase for TGE is usually 2-10 days. Recombinant proteins (r-proteins) produced by TGE can therefore enter the drug development and screening process in a very short time--weeks. With "classical" approaches to protein expression from mammalian cells, it takes months to establish a productive host cell line. This article summarizes efforts in industry and academia to use TGE to produce tens to hundreds of milligrams of r-proteins for either fundamental research or preclinical studies.
Language
  • English
Open access status
green
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https://sonar.ch/global/documents/15957
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