QoI Resistance and Mitochondrial Genetic Structure of Zymoseptoria tritici in Morocco
- Siah, A. Laboratoire Biotechnologie et Gestion des Agents Pathogènes en agriculture, GIS PhyNoPi, Institut Supérieur d'Agriculture, Université Catholique de Lille, 48 Boulevard Vauban, F-59046 Lille cedex, France
- Elbekali, A. Y. Institut National de la Recherche Agronomique, BP 578, Meknès, Morocco
- Ramdani, A. Institut National de la Recherche Agronomique, BP 578, Meknès, Morocco
- Reignault, P. Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV-EA 4492), GIS PhyNoPi, Université du Littoral Côte d'Opale, BP699, F-62228, Calais cedex, France
- Torriani, S. F. F. Institute of Integrative Biology, Plant Pathology, ETH Zurich, CH-8092 Zurich, Switzerland
- Brunner, P. C. Institute of Integrative Biology, Plant Pathology, ETH Zurich, CH-8092 Zurich, Switzerland
- Halama, P. Laboratoire Biotechnologie et Gestion des Agents Pathogènes en agriculture, GIS PhyNoPi, Institut Supérieur d'Agriculture, Université Catholique de Lille
Published in:
- Plant Disease. - Scientific Societies. - 2014, vol. 98, no. 8, p. 1138-1144
English
In total, 230 single-conidial isolates of the fungal wheat pathogen Zymoseptoria tritici (formerly Septoria tritici, teleomorph: Mycosphaerella graminicola) were sampled in Morocco in 2008 and 2010 to assess resistance against quinone outside inhibitors (QoIs), a widely used group of fungicides in wheat pest management. All 134 isolates sampled in 2008 were QoI sensitive. In contrast, 9 of the 96 isolates from the 2010 collection were resistant, suggesting a recent emergence of the resistance. Mitochondrial (mt)DNA-sequence analyses identified four haplotypes among the resistant isolates. Wright's F statistics (FST ) analyses from mtDNA sequences revealed a shallow population structure of Z. tritici within Morocco and a substantial asymmetric gene flow from Europe into Morocco. A phylogenetic reconstruction including Moroccan and European isolates clustered the haplotypes regardless of their geographic origin. The four Moroccan QoI-resistant mitochondrial haplotypes clustered in two distinct clades in the tree topology, suggesting at least two independent origins of the resistance. This study reported, for the first time, the occurrence of QoI-resistant genotypes of Z. tritici in Morocco. Our findings are consistent with the hypothesis that QoI resistance emerged very recently through parallel genetic adaptation in Morocco, although gene flow from Europe cannot be excluded.
- Language
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- English
- Open access status
- hybrid
- Identifiers
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- DOI 10.1094/pdis-10-13-1057-re
- ISSN 0191-2917
- Persistent URL
- https://sonar.ch/global/documents/160721
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