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Whole-genome sequencing for comparative genomics and de novo genome assembly.
Journal article

Whole-genome sequencing for comparative genomics and de novo genome assembly.

  • Benjak A École polytechnique fédérale de Lausanne (EPFL), Global Health Institute, Lausanne, CH-1015, Switzerland.
  • Sala C
  • Hartkoorn RC
  • 2015-03-18
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2015
Computational Biology
Genome
Genome, Bacterial
Genomic Library
Genomics
High-Throughput Nucleotide Sequencing
Mutation
Mycobacterium tuberculosis
English Next-generation sequencing technologies for whole-genome sequencing of mycobacteria are rapidly becoming an attractive alternative to more traditional sequencing methods. In particular this technology is proving useful for genome-wide identification of mutations in mycobacteria (comparative genomics) as well as for de novo assembly of whole genomes. Next-generation sequencing however generates a vast quantity of data that can only be transformed into a usable and comprehensible form using bioinformatics. Here we describe the methodology one would use to prepare libraries for whole-genome sequencing, and the basic bioinformatics to identify mutations in a genome following Illumina HiSeq or MiSeq sequencing, as well as de novo genome assembly following sequencing using Pacific Biosciences (PacBio).
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/163070
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