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Immune serum-activated human macrophages coordinate with eosinophils to immobilize Ascaris suum larvae.
Journal article

Immune serum-activated human macrophages coordinate with eosinophils to immobilize Ascaris suum larvae.

  • Coakley G Department of Immunology and Pathology, Central Clinical School, Monash University, Melbourne, Victoria, Australia.
  • Volpe B Global Health Institute, Swiss Federal Institute of Technology, Lausanne, Switzerland.
  • Bouchery T Department of Immunology and Pathology, Central Clinical School, Monash University, Melbourne, Victoria, Australia.
  • Shah K Global Health Institute, Swiss Federal Institute of Technology, Lausanne, Switzerland.
  • Butler A Department of Immunology and Pathology, Central Clinical School, Monash University, Melbourne, Victoria, Australia.
  • Geldhof P Department of Virology, Parasitology and Immunology, Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
  • Hatherill M Institute of Infectious Disease and Molecular Medicine and Division of Immunology, University of Cape Town, Cape Town, South Africa.
  • Horsnell WGC Institute of Infectious Disease and Molecular Medicine and Division of Immunology, University of Cape Town, Cape Town, South Africa.
  • Esser-von Bieren J Global Health Institute, Swiss Federal Institute of Technology, Lausanne, Switzerland.
  • Harris NL Department of Immunology and Pathology, Central Clinical School, Monash University, Melbourne, Victoria, Australia.
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  • 2020-05-13
Published in:
  • Parasite immunology. - 2020
Ascariasis
Eosinophils
Helminth
Macrophage
antibodies
immune serum
Animals
Antibodies, Helminth
Antibody Formation
Ascariasis
Ascaris lumbricoides
Ascaris suum
Chemokine CCL24
Eosinophils
Humans
Immune Sera
Larva
Leukocyte Count
Macrophages
Mice
Swine
Swine Diseases
Vaccines
English Helminth infection represents a major health problem causing approximately 5 million disability-adjusted life years worldwide. Concerns that repeated anti-helminthic treatment may lead to drug resistance render it important that vaccines are developed but will require increased understanding of the immune-mediated cellular and antibody responses to helminth infection. IL-4 or antibody-activated murine macrophages are known to immobilize parasitic nematode larvae, but few studies have addressed whether this is translatable to human macrophages. In the current study, we investigated the capacity of human macrophages to recognize and attack larval stages of Ascaris suum, a natural porcine parasite that is genetically similar to the human helminth Ascaris lumbricoides. Human macrophages were able to adhere to and trap A suum larvae in the presence of either human or pig serum containing Ascaris-specific antibodies and other factors. Gene expression analysis of serum-activated macrophages revealed that CCL24, a potent eosinophil attractant, was the most upregulated gene following culture with A suum larvae in vitro, and human eosinophils displayed even greater ability to adhere to, and trap, A suum larvae. These data suggest that immune serum-activated macrophages can recruit eosinophils to the site of infection, where they act in concert to immobilize tissue-migrating Ascaris larvae.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/166552
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