Markerless gene deletion system for sphingomonads.

Kaczmarczyk A; Vorholt JA; Francez-Charlot A

doi:10.1128/AEM.07347-11

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Journal article

Markerless gene deletion system for sphingomonads.

Kaczmarczyk A Institute of Microbiology, ETH Zurich, Zurich, Switzerland.
Vorholt JA
Francez-Charlot A

2012-03-20

Published in:

Applied and environmental microbiology. - 2012

English Here, we suggest that natural streptomycin resistance of many sphingomonads resides within rpsL. We constructed a dominant, streptomycin-sensitive rpsL allele and demonstrated its use as a counterselection marker in several sphingomonads. An rpsL-based markerless gene deletion system was developed and validated by deleting four genes in Sphingomonas sp. strain Fr1.

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