Markerless gene deletion system for sphingomonads.
- Kaczmarczyk A Institute of Microbiology, ETH Zurich, Zurich, Switzerland.
- Vorholt JA
- Francez-Charlot A
- 2012-03-20
Published in:
- Applied and environmental microbiology. - 2012
Anti-Bacterial Agents
Biota
Cluster Analysis
DNA, Bacterial
Drug Resistance, Bacterial
Gene Deletion
Genetics, Microbial
Genotype
Molecular Sequence Data
Ribosomal Proteins
Sequence Analysis, DNA
Sphingomonas
Streptomycin
English
Here, we suggest that natural streptomycin resistance of many sphingomonads resides within rpsL. We constructed a dominant, streptomycin-sensitive rpsL allele and demonstrated its use as a counterselection marker in several sphingomonads. An rpsL-based markerless gene deletion system was developed and validated by deleting four genes in Sphingomonas sp. strain Fr1.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1128/AEM.07347-11
- PMID 22427496
- Persistent URL
- https://sonar.ch/global/documents/167769
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