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A separation-integrated cascade reaction to overcome thermodynamic limitations in rare-sugar synthesis.
Journal article

A separation-integrated cascade reaction to overcome thermodynamic limitations in rare-sugar synthesis.

  • 2015-02-18
Published in:
  • Angewandte Chemie (International ed. in English). - 2015
biocatalysis
carbohydrates
enzymes
isomerases
synthetic methods
Carbohydrates
Racemases and Epimerases
Thermodynamics
English Enzyme cascades combining epimerization and isomerization steps offer an attractive route for the generic production of rare sugars starting from accessible bulk sugars but suffer from the unfavorable position of the thermodynamic equilibrium, thus reducing the yield and requiring complex work-up procedures to separate pure product from the reaction mixture. Presented herein is the integration of a multienzyme cascade reaction with continuous chromatography, realized as simulated moving bed chromatography, to overcome the intrinsic yield limitation. Efficient production of D-psicose from sucrose in a three-step cascade reaction using invertase, D-xylose isomerase, and D-tagatose epimerase, via the intermediates D-glucose and D-fructose, is described. This set-up allowed the production of pure psicose (99.9%) with very high yields (89%) and high enzyme efficiency (300 g of D-psicose per g of enzyme).
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/168121
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