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Trapping Transient Protein Species by Genetic Code Expansion.
Journal article

Trapping Transient Protein Species by Genetic Code Expansion.

  • Tinzl M Laboratory of Organic Chemistry, ETH Zürich, Vladimir-Prelog-Weg 1-5/10, 8093, Zürich, Switzerland.
  • Hilvert D Laboratory of Organic Chemistry, ETH Zürich, Vladimir-Prelog-Weg 1-5/10, 8093, Zürich, Switzerland.
  • 2020-08-19
Published in:
  • Chembiochem : a European journal of chemical biology. - 2020
enzyme mechanisms
genetic code expansion
protein complexes
English Nature employs a limited number of genetically encoded amino acids for the construction of functional proteins. By engineering components of the cellular translation machinery, however, it is now possible to genetically encode noncanonical building blocks with tailored electronic and structural properties. The ability to incorporate unique chemical functionality into proteins provides a powerful tool to probe mechanism and create novel function. In this minireview, we highlight several recent studies that illustrate how noncanonical amino acids have been used to capture and characterize reactive intermediates, fine-tune the catalytic properties of enzymes, and stabilize short-lived protein-protein complexes.
Language
  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/168389
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