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Actin dynamics and the Bmp pathway drive apical extrusion of proepicardial cells.

  • Andrés-Delgado L Development of the Epicardium and its Role During Regeneration Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, Spain.
  • Ernst A Institute of Anatomy, University of Bern, 3000 Bern 9, Switzerland.
  • Galardi-Castilla M Development of the Epicardium and its Role During Regeneration Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, Spain.
  • Bazaga D Development of the Epicardium and its Role During Regeneration Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, Spain.
  • Peralta M Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch, France.
  • Münch J Intercellular Signaling in Cardiovascular Development and Disease Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, Spain.
  • González-Rosa JM Development of the Epicardium and its Role During Regeneration Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, Spain.
  • Marques I Institute of Anatomy, University of Bern, 3000 Bern 9, Switzerland.
  • Tessadori F Hubrecht Institute-KNAW and UMC Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands.
  • de la Pompa JL Intercellular Signaling in Cardiovascular Development and Disease Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, Spain.
  • Vermot J Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch, France.
  • Mercader N Development of the Epicardium and its Role During Regeneration Laboratory, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, 28029 Madrid, Spain nadia.mercader@ana.unibe.ch.
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  • 2019-06-09
Published in:
  • Development (Cambridge, England). - 2019
Actomyosin
Bmp
Cell extrusion
Heart development
Proepicardium
Zebrafish
Actins
Animals
Animals, Genetically Modified
Body Patterning
Bone Morphogenetic Proteins
Carrier Proteins
Cell Movement
Embryo, Nonmammalian
Heart
Myocardium
Organogenesis
Pericardium
Signal Transduction
Stem Cells
Zebrafish
Zebrafish Proteins
English The epicardium, the outer mesothelial layer enclosing the myocardium, plays key roles in heart development and regeneration. During embryogenesis, the epicardium arises from the proepicardium (PE), a cell cluster that appears in the dorsal pericardium (DP) close to the venous pole of the heart. Little is known about how the PE emerges from the pericardial mesothelium. Using a zebrafish model and a combination of genetic tools, pharmacological agents and quantitative in vivo imaging, we reveal that a coordinated collective movement of DP cells drives PE formation. We found that Bmp signaling and the actomyosin cytoskeleton promote constriction of the DP, which enables PE cells to extrude apically. We provide evidence that cell extrusion, which has been described in the elimination of unfit cells from epithelia and the emergence of hematopoietic stem cells, is also a mechanism for PE cells to exit an organized mesothelium and fulfil their developmental fate to form a new tissue layer, the epicardium.
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  • English
Open access status
hybrid
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https://sonar.ch/global/documents/170305
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