Back
Unprecedented Sensitivity in a Probe for Monitoring Cathepsin B: Chemiluminescence Microscopy Cell-Imaging of a Natively Expressed Enzyme.
Journal article

Unprecedented Sensitivity in a Probe for Monitoring Cathepsin B: Chemiluminescence Microscopy Cell-Imaging of a Natively Expressed Enzyme.

  • Roth-Konforti ME School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978, Israel.
  • Bauer CR Bioimaging Center, University of Geneva, Geneva, Switzerland.
  • Shabat D School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978, Israel.
  • 2017-10-13
Published in:
  • Angewandte Chemie (International ed. in English). - 2017
cathepsin B
chemiluminescence
dioxetanes
live-cell imaging
molecular probes
3T3 Cells
Animals
Cathepsin B
Cells, Cultured
Fluorescent Dyes
Humans
Luciferases
Luminescent Measurements
Mice
Molecular Structure
Optical Imaging
RAW 264.7 Cells
English Until recently, chemiluminescence cell images could only be obtained using luciferase-activated probes. Moreover, chemiluminescence microscopy cell-imaging has not been demonstrated for natively expressed enzymes like cathepsin B. Herein, we describe the design, synthesis, and evaluation of the first chemiluminescence probe for the detection and imaging of cathepsin B. The probe activation mechanism relies on the release of a dioxetane intermediate, which undergoes chemiexcitation to emit green light with high efficiency under physiological conditions. Using the probe, we obtained clear images of cancerous leukemia and colon cells. This is the first demonstration of chemiluminescence cell images obtained by a probe for a natively expressed endogenous enzyme. We anticipate that the concept presented in this study will be broadly used to develop analogous probes for other important proteases relevant to biomolecular processes.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/170837
Statistics
Document views: 23 File downloads: