Investigating DNA Radiation Damage Using X-Ray Absorption Spectroscopy.
- Czapla-Masztafiak J Paul Scherrer Institut, Villigen, Switzerland; Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland. Electronic address: joanna.czapla-masztafiak@psi.ch.
- Szlachetko J Paul Scherrer Institut, Villigen, Switzerland; Institute of Physics, Jan Kochanowski University in Kielce, Kielce, Poland.
- Milne CJ Paul Scherrer Institut, Villigen, Switzerland.
- Lipiec E Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland.
- Sá J Ångström Laboratory, Department of Chemistry, Uppsala University, Uppsala, Sweden; Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland.
- Penfold TJ Department of Chemistry, Newcastle University, Newcastle upon Tyne, United Kingdom.
- Huthwelker T Paul Scherrer Institut, Villigen, Switzerland.
- Borca C Paul Scherrer Institut, Villigen, Switzerland.
- Abela R Paul Scherrer Institut, Villigen, Switzerland.
- Kwiatek WM Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland.
- 2016-03-31
Published in:
- Biophysical journal. - 2016
English
The biological influence of radiation on living matter has been studied for years; however, several questions about the detailed mechanism of radiation damage formation remain largely unanswered. Among all biomolecules exposed to radiation, DNA plays an important role because any damage to its molecular structure can affect the whole cell and may lead to chromosomal rearrangements resulting in genomic instability or cell death. To identify and characterize damage induced in the DNA sugar-phosphate backbone, in this work we performed x-ray absorption spectroscopy at the P K-edge on DNA irradiated with either UVA light or protons. By combining the experimental results with theoretical calculations, we were able to establish the types and relative ratio of lesions produced by both UVA and protons around the phosphorus atoms in DNA.
- Language
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- English
- Open access status
- bronze
- Identifiers
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- DOI 10.1016/j.bpj.2016.01.031
- PMID 27028640
- Persistent URL
- https://sonar.ch/global/documents/173143
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