Journal article
Occurrence of Brachyspira hyodysenteriae in multiplier pig herds in Switzerland.
- Löbert S Dr. Friederike Zeeh, Clinic for Swine, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, 3001 Bern, Switzerland, Email: friederike.zeeh@vetsuisse.unibe.ch.
- Zimmermann W
- Bürki S
- Frey J
- Nathues H
- Scheer P
- Doherr MG
- Stalder U
- Zeeh F
- 2016-01-14
Published in:
- Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere. - 2016
Prevalence
real-time polymerase chain reaction
risk factors
swine dysentery
Animals
Brachyspira hyodysenteriae
DNA, Bacterial
Gram-Negative Bacterial Infections
Real-Time Polymerase Chain Reaction
Rectum
Swine
Swine Diseases
Switzerland
English
OBJECTIVE
This research was aimed to determine the occurrence of Brachyspira (B.) hyodysenteriae in Swiss multiplier pig herds.
MATERIALS AND METHODS
In a pilot study a direct real-time polymerase chain reaction (PCR) method for B. hyodysenteriae was compared to culture followed by PCR on 106 samples from three herds. Subsequently 40 multiplier herds were epidemiologically characterized and analysed for the presence of B. hyodysenteriae using direct PCR on 1412 rectal swabs. For external validation 20 swabs obtained from two positive conventional herds were analysed.
RESULTS
The comparison of direct PCR with culture followed by PCR resulted in a moderate agreement (kappa index: 0.58). In the two conventional herds, 35% of the samples (7/20) tested positive. Samples from 39 multipliers tested negative. In one multiplier herd, 25% (9/36) of the samples tested PCR positive. Risk factors in the multiplier herd may have been rodents or birds, but not pig purchase.
CONCLUSION AND CLINICAL RELEVANCE
B. hyodysenteriae have been detected in a Swiss multiplier herd, which underlines the threat of potential spread by replacement pigs. Consequently, a Brachyspira monitoring programme was established for Swiss multiplier herds.
This research was aimed to determine the occurrence of Brachyspira (B.) hyodysenteriae in Swiss multiplier pig herds.
MATERIALS AND METHODS
In a pilot study a direct real-time polymerase chain reaction (PCR) method for B. hyodysenteriae was compared to culture followed by PCR on 106 samples from three herds. Subsequently 40 multiplier herds were epidemiologically characterized and analysed for the presence of B. hyodysenteriae using direct PCR on 1412 rectal swabs. For external validation 20 swabs obtained from two positive conventional herds were analysed.
RESULTS
The comparison of direct PCR with culture followed by PCR resulted in a moderate agreement (kappa index: 0.58). In the two conventional herds, 35% of the samples (7/20) tested positive. Samples from 39 multipliers tested negative. In one multiplier herd, 25% (9/36) of the samples tested PCR positive. Risk factors in the multiplier herd may have been rodents or birds, but not pig purchase.
CONCLUSION AND CLINICAL RELEVANCE
B. hyodysenteriae have been detected in a Swiss multiplier herd, which underlines the threat of potential spread by replacement pigs. Consequently, a Brachyspira monitoring programme was established for Swiss multiplier herds.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.15653/TPG-150674
- PMID 26757784
- Persistent URL
- https://sonar.ch/global/documents/173427
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