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Protein-functionalized polymer brushes.
Journal article

Protein-functionalized polymer brushes.

  • Tugulu S Laboratoire des Polymères, Institut des Matériaux, Bâtiment MX-D, and Laboratoire d'Ingénierie des Protéines, Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
  • Arnold A
  • Sielaff I
  • Johnsson K
  • Klok HA
  • 2005-05-10
Published in:
  • Biomacromolecules. - 2005
Polymers
Protein Binding
Proteins
English A new strategy for the preparation of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymerization (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subsequent O(6)-benzylguanine (BG) functionalization. The BG-functionalized brushes are used to chemoselectively immobilize O(6)-alkylguanine-DNA-alkyltransferase (AGT) fusion proteins with a defined orientation and surface density. These protein-modified polymer brushes are attractive candidates for the development of protein microarrays.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/173672
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