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Restoring light sensitivity using tunable near-infrared sensors.
Journal article

Restoring light sensitivity using tunable near-infrared sensors.

  • Nelidova D Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.
  • Morikawa RK Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.
  • Cowan CS Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.
  • Raics Z Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.
  • Goldblum D Department of Ophthalmology, University of Basel, Basel, Switzerland.
  • Scholl HPN Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.
  • Szikra T Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.
  • Szabo A Department of Anatomy, Semmelweis University, Budapest, Hungary.
  • Hillier D Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland. daniel.hillier@iob.ch botond.roska@iob.ch.
  • Roska B Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland. daniel.hillier@iob.ch botond.roska@iob.ch.
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  • 2020-06-06
Published in:
  • Science (New York, N.Y.). - 2020
Animals
Blindness
Disease Models, Animal
Evoked Potentials, Visual
Genetic Engineering
Gold
HEK293 Cells
Humans
Infrared Rays
Mice
Mice, Inbred C57BL
Nanotubes
Photic Stimulation
Rats
Retinal Cone Photoreceptor Cells
Retinal Degeneration
Retinal Ganglion Cells
Sensory Thresholds
Snakes
TRPC Cation Channels
TRPV Cation Channels
Vision, Ocular
Visual Cortex
English Enabling near-infrared light sensitivity in a blind human retina may supplement or restore visual function in patients with regional retinal degeneration. We induced near-infrared light sensitivity using gold nanorods bound to temperature-sensitive engineered transient receptor potential (TRP) channels. We expressed mammalian or snake TRP channels in light-insensitive retinal cones in a mouse model of retinal degeneration. Near-infrared stimulation increased activity in cones, ganglion cell layer neurons, and cortical neurons, and enabled mice to perform a learned light-driven behavior. We tuned responses to different wavelengths, by using nanorods of different lengths, and to different radiant powers, by using engineered channels with different temperature thresholds. We targeted TRP channels to human retinas, which allowed the postmortem activation of different cell types by near-infrared light.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/173824
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