Journal article
ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology
- De Col, Valentina ORCID Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy
- Fuchs, Philippe ORCID Institute of Crop Science and Resource Conservation, University of Bonn, Bonn, Germany
- Nietzel, Thomas ORCID Institute of Crop Science and Resource Conservation, University of Bonn, Bonn, Germany
- Elsässer, Marlene Institute of Crop Science and Resource Conservation, University of Bonn, Bonn, Germany
- Voon, Chia Pao ORCID School of Biological Sciences, University of Hong Kong, Hong Kong, China
- Candeo, Alessia ORCID Dipartimento di Fisica, Politecnico di Milano, Milano, Italy
- Seeliger, Ingo Institute of Crop Science and Resource Conservation, University of Bonn, Bonn, Germany
- Fricker, Mark D ORCID Department of Plant Sciences, University of Oxford, Oxford, United Kingdom
- Grefen, Christopher ORCID Centre for Plant Molecular Biology, Developmental Genetics, University of Tübingen, Tübingen, Germany
- Møller, Ian Max Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
- Bassi, Andrea ORCID Dipartimento di Fisica, Politecnico di Milano, Milano, Italy
- Lim, Boon Leong ORCID State Key Laboratory of Agrobiotechnology, Chinese University of Hong Kong, Hong Kong, China
- Zancani, Marco ORCID Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy
- Meyer, Andreas J ORCID Bioeconomy Science Center, Forschungszentrum Jülich, Jülich, Germany
- Costa, Alex ORCID Dipartimento di Bioscienze, Università degli Studi di Milano, Milano, Italy
- Wagner, Stephan ORCID Institute of Crop Science and Resource Conservation, University of Bonn, Bonn, Germany
- Schwarzländer, Markus ORCID Bioeconomy Science Center, Forschungszentrum Jülich, Jülich, Germany
- 2017-7-18
Published in:
- eLife. - eLife Sciences Publications, Ltd. - 2017, vol. 6
English
Growth and development of plants is ultimately driven by light energy captured through photosynthesis. ATP acts as universal cellular energy cofactor fuelling all life processes, including gene expression, metabolism, and transport. Despite a mechanistic understanding of ATP biochemistry, ATP dynamics in the living plant have been largely elusive. Here, we establish MgATP2- measurement in living plants using the fluorescent protein biosensor ATeam1.03-nD/nA. We generate Arabidopsis sensor lines and investigate the sensor in vitro under conditions appropriate for the plant cytosol. We establish an assay for ATP fluxes in isolated mitochondria, and demonstrate that the sensor responds rapidly and reliably to MgATP2- changes in planta. A MgATP2- map of the Arabidopsis seedling highlights different MgATP2- concentrations between tissues and within individual cell types, such as root hairs. Progression of hypoxia reveals substantial plasticity of ATP homeostasis in seedlings, demonstrating that ATP dynamics can be monitored in the living plant.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.7554/eLife.26770
- ISSN 2050-084X
- Persistent URL
- https://sonar.ch/global/documents/176950
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