Back
Beneficial and detrimental impact of transplanted canine adipose-derived stem cells in a virus-induced demyelinating mouse model.
Journal article

Beneficial and detrimental impact of transplanted canine adipose-derived stem cells in a virus-induced demyelinating mouse model.

  • Hansmann F Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, 30559, Hannover, Germany; Center for Systems Neuroscience, Bünteweg 2, 30559, Hannover, Germany.
  • Jungwirth N Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, 30559, Hannover, Germany; Center for Systems Neuroscience, Bünteweg 2, 30559, Hannover, Germany.
  • Zhang N Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, 30559, Hannover, Germany; Center for Systems Neuroscience, Bünteweg 2, 30559, Hannover, Germany.
  • Skripuletz T Department of Neurology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
  • Stein VM Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, Bünteweg 9, 30559, Hannover, Germany; Division of Neurology, Department of Clinical Veterinary Sciences, Vetsuisse Faculty, University of Bern, Länggassstrasse 128, 3012, Bern, Switzerland.
  • Tipold A Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, Bünteweg 9, 30559, Hannover, Germany; Center for Systems Neuroscience, Bünteweg 2, 30559, Hannover, Germany.
  • Stangel M Department of Neurology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany; Center for Systems Neuroscience, Bünteweg 2, 30559, Hannover, Germany.
  • Baumgärtner W Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, 30559, Hannover, Germany; Center for Systems Neuroscience, Bünteweg 2, 30559, Hannover, Germany. Electronic address: Wolfgang.Baumgaertner@tiho-hannover.de.
Show more…
  • 2018-08-07
Published in:
  • Veterinary immunology and immunopathology. - 2018
Axonal damage
Canine adipose-derived stem cells
Demyelination
Lung priming
Multiple sclerosis
Theiler’s murine encephalomyelitis
Adipose Tissue
Animals
CD4-Positive T-Lymphocytes
Cardiovirus Infections
Demyelinating Diseases
Disease Models, Animal
Distemper Virus, Canine
Dogs
Infusions, Intravenous
Lung
Mice
Multiple Sclerosis
Stem Cell Transplantation
Stem Cells
Theilovirus
English In recent years stem cell therapies have been broadly applied in various disease models specifically immune mediated and degenerative diseases. Whether adipose-derived stem cells might represent a useful therapeutic option in virus-triggered central nervous system diseases has not been investigated so far. Theiler's murine encephalomyelitis (TME) and canine distemper encephalitis are established, virus-mediated animal models sharing many similarities with multiple sclerosis (MS). Canine adipose-derived stem cells (ASC) were selected since dogs might serve as an important translational model for further therapeutic applications. The aim of the present study was to investigate whether canine ASC influence clinical signs, axonal damage, demyelination and inflammation during TME. ASC were transplanted intravenously (iv) or intra-cerebroventricularly (icv) at 7 (early) or 42 (late) days post infection (dpi) in TME virus (TMEV) infected mice. TMEV/ASC iv animals transplanted at 7dpi displayed a transient clinical deterioration in rotarod performance compared to TMEV/control animals. Worsening of clinical signs was associated with significantly increased numbers of microglia/macrophages and demyelination in the spinal cord. In contrast, late transplantation had no influence on clinical findings of TMEV-infected animals. However, late TMEV/ASC iv transplanted animals showed reduced axonal damage compared to TMEV/control animals. Screening of spinal cord and peripheral organs for transplanted ASC revealed no positive cells. Surprisingly, iv transplanted animals showed pulmonary follicular aggregates consisting of T- and B-lymphocytes. Thus, our data suggest that following intravenous application, the lung as priming organ for lymphocytes seems to play a pivotal role in the pathogenesis of TME. Consequences of T-lymphocyte priming in the lung depend on the disease phase and may be responsible for disease modifying effects of ASC.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/178559
Statistics
Document views: 13 File downloads: