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Blood monitoring of perfluorocarbon compounds (F-tert-butylcyclohexane, perfluoromethyldecalin and perfluorodecalin) by headspace-gas chromatography-tandem mass spectrometry.
Journal article

Blood monitoring of perfluorocarbon compounds (F-tert-butylcyclohexane, perfluoromethyldecalin and perfluorodecalin) by headspace-gas chromatography-tandem mass spectrometry.

  • Giuliani N Forensic Toxicology and Chemistry Unit University Centre of Legal Medicine Lausanne - Geneva, CH-1011 Lausanne, Switzerland.
  • Saugy M Swiss Laboratory for Doping Analyses University Centre of Legal Medicine Lausanne - Geneva, CH-1066 Epalinges, Switzerland.
  • Augsburger M Forensic Toxicology and Chemistry Unit University Centre of Legal Medicine Lausanne - Geneva, CH-1011 Lausanne, Switzerland.
  • Varlet V Forensic Toxicology and Chemistry Unit University Centre of Legal Medicine Lausanne - Geneva, CH-1011 Lausanne, Switzerland. Electronic address: vincent.varlet@chuv.ch.
  • 2015-10-11
Published in:
  • Talanta. - 2015
F-tert-butylcyclohexane
Perfluoro(methyldecalin)
Perfluorocarbons
Perfluorodecalin
Tandem mass spectrometry
Cyclohexanes
Fluorocarbons
Gas Chromatography-Mass Spectrometry
Humans
Reproducibility of Results
Tandem Mass Spectrometry
English A headspace-gas chromatography-tandem mass spectrometry (HS-GC-MS/MS) method for the trace measurement of perfluorocarbon compounds (PFCs) in blood was developed. Due to oxygen carrying capabilities of PFCs, application to doping and sports misuse is speculated. This study was therefore extended to perform validation methods for F-tert-butylcyclohexane (Oxycyte(®)), perfluoro(methyldecalin) (PFMD) and perfluorodecalin (PFD). The limit of detection of these compounds was established and found to be 1.2 µg/mL blood for F-tert-butylcyclohexane, 4.9 µg/mL blood for PFMD and 9.6 µg/mL blood for PFD. The limit of quantification was assumed to be 12 µg/mL blood (F-tert-butylcyclohexane), 48 µg/mL blood (PFMD) and 96 µg/mL blood (PFD). HS-GC-MS/MS technique allows detection from 1000 to 10,000 times lower than the estimated required dose to ensure a biological effect for the investigated PFCs. Thus, this technique could be used to identify a PFC misuse several hours, maybe days, after the injection or the sporting event. Clinical trials with those compounds are still required to evaluate the validation parameters with the calculated estimations.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/181399
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