Cuticular Defects in Oryza sativa ATP-binding Cassette Transporter G31 Mutant Plants Cause Dwarfism, Elevated Defense Responses and Pathogen Resistance.
- Garroum I University of Lausanne, Department of Plant Molecular Biology, Biophore Building, CH-1015 Lausanne, Switzerland.
- Bidzinski P INRA, UMR-BGPI TA A-54/K, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France Present address: INRA, SupAgro, UMR-BPMP, Bat. 7, 2 place Pierre Viala, 34060 Montpellier, Cedex 2, France.
- Daraspe J University of Lausanne, Electron Microscopy Facility, Biophore Building, CH-1015 Lausanne, Switzerland.
- Mucciolo A University of Lausanne, Electron Microscopy Facility, Biophore Building, CH-1015 Lausanne, Switzerland.
- Humbel BM University of Lausanne, Electron Microscopy Facility, Biophore Building, CH-1015 Lausanne, Switzerland.
- Morel JB INRA, UMR-BGPI TA A-54/K, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France.
- Nawrath C University of Lausanne, Department of Plant Molecular Biology, Biophore Building, CH-1015 Lausanne, Switzerland christiane.nawrath@unil.ch.
- 2016-04-29
Published in:
- Plant & cell physiology. - 2016
ABC transporter
Cuticle
Cutin
Magnaporthe
Pathogen defense
Rice
ATP-Binding Cassette Transporters
Disease Resistance
Gene Expression Regulation, Plant
Magnaporthe
Membrane Lipids
Mutation
Oryza
Phenotype
Plant Diseases
Plant Epidermis
Plant Proteins
English
The cuticle covers the surface of the polysaccharide cell wall of leaf epidermal cells and forms an essential diffusion barrier between plant and environment. Homologs of the ATP-binding cassette (ABC) transporter AtABCG32/HvABCG31 clade are necessary for the formation of a functional cuticle in both monocots and dicots. Here we characterize the osabcg31 knockout mutant and hairpin RNA interference (RNAi)-down-regulated OsABCG31 plant lines having reduced plant growth and a permeable cuticle. The reduced content of cutin in leaves and structural alterations in the cuticle and at the cuticle-cell wall interface in plants compromised in OsABCG31 expression explain the cuticle permeability. Effects of modifications of the cuticle on plant-microbe interactions were evaluated. The cuticular alterations in OsABCG31-compromised plants did not cause deficiencies in germination of the spores or the formation of appressoria of Magnaporthe oryzae on the leaf surface, but a strong reduction of infection structures inside the plant. Genes involved in pathogen resistance were constitutively up-regulated in OsABCG31-compromised plants, thus being a possible cause of the resistance to M. oryzae and the dwarf growth phenotype. The findings show that in rice an abnormal cuticle formation may affect the signaling of plant growth and defense.
- Language
-
- English
- Open access status
- bronze
- Identifiers
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- DOI 10.1093/pcp/pcw066
- PMID 27121976
- Persistent URL
- https://sonar.ch/global/documents/181528
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