Journal article
Comparison of double-locus sequence typing (DLST) and multilocus sequence typing (MLST) for the investigation of Pseudomonas aeruginosa populations.
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Cholley P
Infection Control Department, UMR Chrono-environnement (CNRS), University Hospital of Besançon, Besançon, France.
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Stojanov M
Service of Hospital Preventive Medicine, Lausanne University Hospital, Lausanne, Switzerland.
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Hocquet D
Infection Control Department, UMR Chrono-environnement (CNRS), University Hospital of Besançon, Besançon, France.
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Thouverez M
Infection Control Department, UMR Chrono-environnement (CNRS), University Hospital of Besançon, Besançon, France.
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Bertrand X
Infection Control Department, UMR Chrono-environnement (CNRS), University Hospital of Besançon, Besançon, France.
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Blanc DS
Service of Hospital Preventive Medicine, Lausanne University Hospital, Lausanne, Switzerland. Electronic address: Dominique.Blanc@chuv.ch.
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Published in:
- Diagnostic microbiology and infectious disease. - 2015
English
Reliable molecular typing methods are necessary to investigate the epidemiology of bacterial pathogens. Reference methods such as multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) are costly and time consuming. Here, we compared our newly developed double-locus sequence typing (DLST) method for Pseudomonas aeruginosa to MLST and PFGE on a collection of 281 isolates. DLST was as discriminatory as MLST and was able to recognize "high-risk" epidemic clones. Both methods were highly congruent. Not surprisingly, a higher discriminatory power was observed with PFGE. In conclusion, being a simple method (single-strand sequencing of only 2 loci), DLST is valuable as a first-line typing tool for epidemiological investigations of P. aeruginosa. Coupled to a more discriminant method like PFGE or whole genome sequencing, it might represent an efficient typing strategy to investigate or prevent outbreaks.
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Language
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Open access status
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closed
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Identifiers
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Persistent URL
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https://sonar.ch/global/documents/181595
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