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A high resolution LC-MS targeted method for the concomitant analysis of 11 contraceptive progestins and 4 steroids.
Journal article

A high resolution LC-MS targeted method for the concomitant analysis of 11 contraceptive progestins and 4 steroids.

  • Laszlo CF Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole polytechnique fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland. Electronic address: csaba.laszlo@epfl.ch.
  • Paz Montoya J Proteomics Core Facility, École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. Electronic address: jonathan.pazmontoya@epfl.ch.
  • Shamseddin M Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole polytechnique fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland. Electronic address: marie.shamseddin@epfl.ch.
  • De Martino F Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole polytechnique fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland. Electronic address: fabio.demartino@epfl.ch.
  • Beguin A Clinical Chemistry Laboratory, Centre Hospitalier Universitaire Vaudois (CHUV), CH-1011, Lausanne, Switzerland. Electronic address: Alexandre.Beguin@chuv.ch.
  • Nellen R Clinical Chemistry Laboratory, Centre Hospitalier Universitaire Vaudois (CHUV), CH-1011, Lausanne, Switzerland. Electronic address: nellenr@gmail.com.
  • Bruce SJ Clinical Chemistry Laboratory, Centre Hospitalier Universitaire Vaudois (CHUV), CH-1011, Lausanne, Switzerland. Electronic address: Steve.Bruce@chuv.ch.
  • Moniatte M Proteomics Core Facility, École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. Electronic address: marc.moniatte@epfl.ch.
  • Henry H Département formation et recherche, Centre Hospitalier Universitaire Vaudois (CHUV), CH-1011, Lausanne, Switzerland. Electronic address: Hugues.Henry@chuv.ch.
  • Brisken C Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole polytechnique fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland. Electronic address: cathrin.brisken@epfl.ch.
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  • 2019-08-07
Published in:
  • Journal of pharmaceutical and biomedical analysis. - 2019
Contraceptives
HRT
Hormones
LC- HRMS
Progestins
Steroids
Animals
Chromatography, Liquid
Contraceptive Agents
Female
Humans
Mice
Mice, Inbred NOD
Progestins
Steroids
Tandem Mass Spectrometry
English In the context of hormonal contraception and hormone replacement therapy (HRT), many women are exposed to exogenous hormones. Current use of hormonal contraception with combined ethinyl estradiol and different progestins bestows a breast cancer relative risk (RR) of 1.2- while combined HRT has a RR of 2. Although these exposures present an important public health issue, little is known about the effects of individual progestins on the breast and other tissues. Increasing availability of large scale biobanks, high throughput analyses and data management tools enable ever expanding, sophisticated population studies. In order to address the impact of distinct progestins on various health indicators, it is desirable to accurately quantify progestins in clinical samples. Here we have developed and validated a high resolution liquid chromatography mass spectrometry (LC-MS) targeted method for the simultaneous quantification of 11 synthetic progestins widely used in oral contraceptives, gestodene, levonorgestrel, etonogestrel, chlormadinone acetate, cyproterone acetate, drospirenone, desacetyl norgestimate, medroxyprogesterone acetate, norethindrone, dienogest, nomegestrol acetate, and 4 endogenous steroid hormones, progesterone, testosterone, androstenedione, and cortisol in blood samples. This highly specific quantitative analysis with high resolution Orbitrap technology detects and quantifies 15 compounds using their internal standard counterparts in a single 12 min LC-MS run. Sensitivity is attained by the use of the instrument in targeted selected ion monitoring mode. Lower limit of quantitation ranges from 2.4 pg/ml for drospirenone to 78.1 pg/ml for chlormadinone acetate. The method provides comprehensive progestin panel measurements with as little as 50 μl of murine or human plasma.
Language
  • English
Open access status
hybrid
Identifiers
Persistent URL
https://sonar.ch/global/documents/182414
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