Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome.
- Lesche D University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, INO F, 3010 Bern, Switzerland ; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
- Geyer R University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, INO F, 3010 Bern, Switzerland.
- Lienhard D University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, INO F, 3010 Bern, Switzerland.
- Nakas CT University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, INO F, 3010 Bern, Switzerland ; Laboratory of Biometry, University of Thessaly, Volos, Greece.
- Diserens G Departments of Clinical Research and Radiology (AMSM), Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland ; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
- Vermathen P Departments of Clinical Research and Radiology (AMSM), Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
- Leichtle AB University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, INO F, 3010 Bern, Switzerland.
- 2016-10-13
Published in:
- Metabolomics : Official journal of the Metabolomic Society. - 2016
English
BACKGROUND
Centrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs.
AIM
Determine whether routinely used plasma centrifugation protocols (1500×g 10 min; 3000×g 5 min) influence non-targeted metabolomic analyses.
METHODS
Nuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared with cell count measurements.
RESULTS
Besides significant differences in platelet count, we identified substantial alterations in NMR and HRMS data related to the different centrifugation protocols.
CONCLUSION
Already minor differences in plasma centrifugation can significantly influence metabolomic patterns and potentially bias metabolomics studies.
Centrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs.
AIM
Determine whether routinely used plasma centrifugation protocols (1500×g 10 min; 3000×g 5 min) influence non-targeted metabolomic analyses.
METHODS
Nuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared with cell count measurements.
RESULTS
Besides significant differences in platelet count, we identified substantial alterations in NMR and HRMS data related to the different centrifugation protocols.
CONCLUSION
Already minor differences in plasma centrifugation can significantly influence metabolomic patterns and potentially bias metabolomics studies.
- Language
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- English
- Open access status
- hybrid
- Identifiers
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- DOI 10.1007/s11306-016-1109-3
- PMID 27729833
- Persistent URL
- https://sonar.ch/global/documents/185096
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