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Process for Continuous Fab Production by Digestion of IgG.
Journal article

Process for Continuous Fab Production by Digestion of IgG.

  • Ulmer N Department of Chemistry and Applied Bioscience, ETH Zurich, Vladimir-Prelog-Weg 1, 8093, Zurich, Switzerland.
  • Ristanovic D Department of Chemistry and Applied Bioscience, ETH Zurich, Vladimir-Prelog-Weg 1, 8093, Zurich, Switzerland.
  • Morbidelli M Department of Chemistry and Applied Bioscience, ETH Zurich, Vladimir-Prelog-Weg 1, 8093, Zurich, Switzerland.
  • 2019-06-07
Published in:
  • Biotechnology journal. - 2019
Enzyme reactor
MCSGP
continuous chromatography
digestion
Antibodies, Monoclonal
Countercurrent Distribution
Humans
Immunoglobulin Fab Fragments
Immunoglobulin G
Models, Chemical
Papain
English Intensified processing and end-to-end integrated continuous manufacturing are increasingly being considered in bioprocessing as an alternative to the current batch-based technologies. Similar approaches can also be used at later stages of the production chain, such as in the post-translational modifications that are often considered for therapeutic proteins. In this work, a process to intensify the enzymatic digestion of immunoglobulin G (IgG) and the purification of the resulting Fab fragment is developed. The process consists of the integration of a continuous packed-bed reactor into a multicolumn chromatographic process. The integration is realized through the development of a novel multicolumn countercurrent solvent gradient purification (MCSGP) process, which, by adding a third column to the classical two-column MCSGP process, allows for continuous loading and then straight-through processing of the mixture leaving the reactor.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/185210
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