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An analytical method to control the surface density and stability of DNA-gold nanoparticles for an optimized biosensor.
Journal article

An analytical method to control the surface density and stability of DNA-gold nanoparticles for an optimized biosensor.

  • Hwu S Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, 8092, Zurich, Switzerland.
  • Garzuel M Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, 8092, Zurich, Switzerland.
  • Forró C Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, 8092, Zurich, Switzerland.
  • Ihle SJ Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, 8092, Zurich, Switzerland.
  • Reichmuth AM Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, 8092, Zurich, Switzerland.
  • Kurdzesau F Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, 8092, Zurich, Switzerland.
  • Vörös J Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, 8092, Zurich, Switzerland. Electronic address: janos.voros@biomed.ee.ethz.ch.
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  • 2019-12-03
Published in:
  • Colloids and surfaces. B, Biointerfaces. - 2020
Colloid stability
DNA functionalization
Gold nanoparticle
Multifunctional colloids
Quantifying ligand density
Snap freezing
English DNA functionalized gold nanoparticles (DNA-AuNPs) have shown great potential for biosensing as they combine the excellent optical properties of gold nanoparticles and the molecular recognition function of DNA. Since the DNA density determines the assay performance and the stability of the conjugate, a precise control of the surface density of DNA-AuNP is crucial for an optimized biosensor. Here we report a simple assay for quantifying multiple unlabeled DNAs on AuNPs. The assay relies on potassium cyanide (KCN) to first dissolve the AuNPs, which then releases surface bound DNA for quantification through a double-stranded DNA dye. Using this analytical quantification method, we investigated several strategies to control the surface density of DNA-AuNPs. Besides the precise control of DNA density, the stability of DNA-AuNPs after conjugation is also important in developing a biosensor with optimal performance. Without proper storing conditions, DNA-AuNPs are unstable and aggregate over time. To overcome this problem, we developed a long-term storage solution to ensure the stability and quality of DNA-AuNPs after conjugation which would benefit any DNA-AuNP-based biosensor.
Language
  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/185284
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