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Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle.

  • Keller P Institute of Microbiology, Department of Biology, ETH Zurich, 8093, Zurich, Switzerland.
  • Noor E Institute of Molecular Systems Biology, Department of Biology, ETH Zurich, 8093, Zurich, Switzerland.
  • Meyer F Institute of Microbiology, Department of Biology, ETH Zurich, 8093, Zurich, Switzerland.
  • Reiter MA Institute of Microbiology, Department of Biology, ETH Zurich, 8093, Zurich, Switzerland.
  • Anastassov S Institute of Microbiology, Department of Biology, ETH Zurich, 8093, Zurich, Switzerland.
  • Kiefer P Institute of Microbiology, Department of Biology, ETH Zurich, 8093, Zurich, Switzerland.
  • Vorholt JA Institute of Microbiology, Department of Biology, ETH Zurich, 8093, Zurich, Switzerland. jvorholt@ethz.ch.
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  • 2020-10-27
Published in:
  • Nature communications. - 2020
Bacterial Proteins
Escherichia coli
Escherichia coli Proteins
Fructose-Bisphosphatase
Metabolic Engineering
Methanol
Ribulosephosphates
Triose-Phosphate Isomerase
English Methanol is a biotechnologically promising substitute for food and feed substrates since it can be produced renewably from electricity, water and CO2. Although progress has been made towards establishing Escherichia coli as a platform organism for methanol conversion via the energy efficient ribulose monophosphate (RuMP) cycle, engineering strains that rely solely on methanol as a carbon source remains challenging. Here, we apply flux balance analysis to comprehensively identify methanol-dependent strains with high potential for adaptive laboratory evolution. We further investigate two out of 1200 candidate strains, one with a deletion of fructose-1,6-bisphosphatase (fbp) and another with triosephosphate isomerase (tpiA) deleted. In contrast to previous reported methanol-dependent strains, both feature a complete RuMP cycle and incorporate methanol to a high degree, with up to 31 and 99% fractional incorporation into RuMP cycle metabolites. These strains represent ideal starting points for evolution towards a fully methylotrophic lifestyle.
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  • English
Open access status
gold
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https://sonar.ch/global/documents/185300
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