Back
Antiviral activity of K22 against members of the order Nidovirales.
Journal article

Antiviral activity of K22 against members of the order Nidovirales.

  • Rappe JCF Institute for Virology and Immunology IVI, Mittelhäusern and Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland; Department of Infectious Diseases and Pathobiology, University of Bern, Switzerland. Electronic address: julie.rappe@crick.ac.uk.
  • de Wilde A Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: A.H.de_Wilde@lumc.nl.
  • Di H Department of Biology, 623 Petit Science Center, Georgia State University, 161 Jesse Hill Jr Drive, Atlanta, GA 30303, United States. Electronic address: hdi1@gsu.edu.
  • Müller C Institute of Medical Virology, Justus Liebig University, Giessen, Germany. Electronic address: Christin.Mueller@viro.med.uni-giessen.de.
  • Stalder H Institute for Virology and Immunology IVI, Mittelhäusern and Bern, Switzerland; Department of Infectious Diseases and Pathobiology, University of Bern, Switzerland. Electronic address: hanspeter.stalder@vetsuisse.unibe.ch.
  • V'kovski P Institute for Virology and Immunology IVI, Mittelhäusern and Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland; Department of Infectious Diseases and Pathobiology, University of Bern, Switzerland. Electronic address: philip.vkovski@vetsuisse.unibe.ch.
  • Snijder E Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: e.j.snijder@lumc.nl.
  • Brinton MA Department of Biology, 623 Petit Science Center, Georgia State University, 161 Jesse Hill Jr Drive, Atlanta, GA 30303, United States. Electronic address: mbrinton@gsu.edu.
  • Ziebuhr J Institute of Medical Virology, Justus Liebig University, Giessen, Germany. Electronic address: John.Ziebuhr@viro.med.uni-giessen.de.
  • Ruggli N Institute for Virology and Immunology IVI, Mittelhäusern and Bern, Switzerland; Department of Infectious Diseases and Pathobiology, University of Bern, Switzerland. Electronic address: nicolas.ruggli@ivi.admin.ch.
  • Thiel V Institute for Virology and Immunology IVI, Mittelhäusern and Bern, Switzerland; Department of Infectious Diseases and Pathobiology, University of Bern, Switzerland. Electronic address: volker.thiel@vetsuisse.unibe.ch.
Show more…
  • 2018-01-17
Published in:
  • Virus research. - 2018
Antiviral drug
Double membrane vesicles
K22
Nidoviruses
Replication organelles
Animals
Antiviral Agents
Arterivirus
Benzamides
Carps
Cell Line
Chlorocebus aethiops
Coronaviridae
Epithelial Cells
Equartevirus
Mesocricetus
Piperidines
Porcine respiratory and reproductive syndrome virus
RNA, Double-Stranded
RNA, Viral
Torovirus
Virus Replication
English Recently, a novel antiviral compound (K22) that inhibits replication of a broad range of animal and human coronaviruses was reported to interfere with viral RNA synthesis by impairing double-membrane vesicle (DMV) formation (Lundin et al., 2014). Here we assessed potential antiviral activities of K22 against a range of viruses representing two (sub)families of the order Nidovirales, the Arteriviridae (porcine reproductive and respiratory syndrome virus [PRRSV], equine arteritis virus [EAV] and simian hemorrhagic fever virus [SHFV]), and the Torovirinae (equine torovirus [EToV] and White Bream virus [WBV]). Possible effects of K22 on nidovirus replication were studied in suitable cell lines. K22 concentrations significantly decreasing infectious titres of the viruses included in this study ranged from 25 to 50 μM. Reduction of double-stranded RNA intermediates of viral replication in nidovirus-infected cells treated with K22 confirmed the anti-viral potential of K22. Collectively, the data show that K22 has antiviral activity against diverse lineages of nidoviruses, suggesting that the inhibitor targets a critical and conserved step during nidovirus replication.
Language
  • English
Open access status
hybrid
Identifiers
Persistent URL
https://sonar.ch/global/documents/185371
Statistics
Document views: 19 File downloads: