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Insulin regulates the expression of the insulin-like growth factor binding protein 2 mRNA in rat hepatocytes.
Journal article

Insulin regulates the expression of the insulin-like growth factor binding protein 2 mRNA in rat hepatocytes.

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  • 1990-09-01
Published in:
  • Molecular endocrinology (Baltimore, Md.). - 1990
Animals
Carrier Proteins
Cells, Cultured
Dose-Response Relationship, Drug
Gene Expression Regulation
Growth Hormone
Insulin
Insulin-Like Growth Factor Binding Proteins
Liver
Male
Nucleic Acid Hybridization
RNA, Messenger
Rats
Rats, Inbred Strains
English The goal of this study was to find out whether GH or insulin regulate the mRNA expression of the fetal binding protein of insulin-like growth factor (IGFBP-2). Primary hepatocytes from adult rats were used as a test system. IGFBP-2 mRNA was abundant in cells cultured in the absence of hormones and markedly reduced in cultures containing insulin. Addition of GH had no effect on IGFBP-2 mRNA levels although the cells are responsive to GH as demonstrated by a GH mediated elevation of IGF l mRNA levels. Half-maximal down-regulation of IGFBP-2 mRNA levels occurred at an insulin concentration of 1 to 2 x 10(-10) M. The finding that insulin is a potent negative regulator of hepatic IGFBP-2 mRNA levels suggests a physiologically important regulatory link between the two hormones insulin and IGF l.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/185391
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