Journal article
Expression of a human insulin-like growth factor II cDNA in NIH-3T3 cells.
- Buergisser DM Department of Biochemistry, University of Zürich, Switzerland.
- Roth BV
- Luethi C
- Gerber HP
- Honegger A
- Humbel RE
- 1990-06-29
Published in:
- Biochemical and biophysical research communications. - 1990
Animals
Cell Line
Cloning, Molecular
DNA
Gene Expression
Genetic Vectors
Humans
In Vitro Techniques
Insulin-Like Growth Factor II
Mice
Molecular Weight
Radioligand Assay
Somatomedins
English
Recombinant human insulin-like growth factor II (IGF-II) was produced in NIH-3T3 cells transfected with a plasmid containing a construct encoding the signal peptide and the sequence of mature human IGF II. Successfully transfected clones secreted correctly processed recombinant human IGF II at rates of about 100ng per 24 hours per 10(6) cells. The biological activity of the purified recombinant human IGF II exhibited similar potencies as standard human IGF II isolated from serum in radio-receptorassays as well as in thymidine incorporation assays.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/0006-291x(90)91968-x
- PMID 2363726
- Persistent URL
- https://sonar.ch/global/documents/185437
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