Journal article
Protein immobilization technology for flow biocatalysis.
- Romero-Fernández M School of Chemistry, University of Nottingham, University Park, NG7 2RD, Nottingham, UK.
- Paradisi F School of Chemistry, University of Nottingham, University Park, NG7 2RD, Nottingham, UK; Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012, Bern, Switzerland. Electronic address: francesca.paradisi@dcb.unibe.ch.
- 2019-12-23
Published in:
- Current opinion in chemical biology. - 2020
English
Enzymatic immobilization has been at the forefront of applied biocatalysis as it enables convenient isolation and reuse of the catalyst if the target reaction is conducted in batch, and it has opened up significant opportunities to conduct biocatalysis in continuous mode. Over the last few years, an array of techniques to immobilize enzymes have been developed, spanning from covalent multipoint attachment to noncovalent electrostatic strategies to rational architecture to suitably orient the enzyme(s). In addition, new materials have been adapted to support biological catalysts. Here, we discuss the advances of the last two years in enzyme immobilization for continuous flow applications.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1016/j.cbpa.2019.11.008
- PMID 31865258
- Persistent URL
- https://sonar.ch/global/documents/185460
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