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Journal article

Expression cloning of rat renal Na+/SO4(2-) cotransport.

  • 1993-09-01
Published in:
  • Proceedings of the National Academy of Sciences of the United States of America. - 1993
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Carrier Proteins
Cation Transport Proteins
Cloning, Molecular
DNA
Female
Gene Library
Kidney Cortex
Kinetics
Molecular Sequence Data
Oligonucleotides, Antisense
Oocytes
Organ Specificity
Poly A
Protein Biosynthesis
Protein Conformation
RNA
RNA, Messenger
Rats
Sodium
Sodium Sulfate Cotransporter
Sulfates
Symporters
Xenopus laevis
English Injection of rat kidney cortex mRNA into Xenopus laevis oocytes leads to a stimulation of Na(+)-dependent SO4(2-) uptake. Based on this information, we have isolated from a corresponding library a cDNA (NaSi-1) that is most likely related to a Na+/SO4(2-) cotransport system. NaSi-1 cRNA leads in a time- and dose-dependent manner to specific stimulation of Na(+)-dependent SO4(2-) uptake in oocytes. The apparent affinity constants of the NaSi-1 cRNA-expressed transport resemble those of Na+/SO4(2-) cotransport in brush-border membrane. The NaSi-1 cDNA contains 2239 bp [including a poly(A) tail] and encodes a protein of 595 amino acids (66.05 kDa); the hydropathy profile suggests at least eight membrane-spanning regions. In vitro translation of NaSi-1 cRNA results in a protein of the expected size and suggests glycosylation. Northern blot analysis shows signals of 2.3 and 2.9 kb in kidney (more abundant in cortex than in papilla/medulla) and in mucosa of small intestine of rats. The above data indicate that we have structurally identified a membrane protein involved in renal and small-intestinal brush-border membrane Na+/SO4(2-) cotransport.
Language
  • English
Open access status
bronze
Identifiers
Persistent URL
https://sonar.ch/global/documents/185529
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