Journal article
Development of a gas chromatography-mass spectrometry method for determination of ketamine in plasma and its application to human samples.
- Feng N Institute of Clinical Chemistry, University Hospital Zurich, Switzerland.
- Vollenweider FX
- Minder EI
- Rentsch K
- Grampp T
- Vonderschmitt DJ
- 1995-02-01
Published in:
- Therapeutic drug monitoring. - 1995
Adult
Chromatography, High Pressure Liquid
Female
Gas Chromatography-Mass Spectrometry
Humans
Ketamine
Male
Psychoses, Substance-Induced
English
A sensitive and precise gas chromatography-mass spectrometry method with selected ion monitoring has been developed for identification and quantification of the phencyclidine derivative ketamine in human plasma. The assay is based on an alkaline extraction from aqueous to organic solvent from plasma and an efficient gas chromatographic separation on a DB-5 capillary column. The analytical procedure has a coefficient of variation of 0.7-6.2% and from 1.3 to 8.7% within-day and from day-to-day analysis, respectively. The low level of sensitivity was 10 ng/ml. It was used to measure low plasma concentrations in volunteers during ketamine-induced experimental psychosis. The method is not enantio selective.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1097/00007691-199502000-00016
- PMID 7725385
- Persistent URL
- https://sonar.ch/global/documents/185534
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