Journal article
Inclusion of an upstream transcriptional terminator in phage display vectors abolishes background expression of toxic fusions with coat protein g3p.
- Krebber A Biochemisches Institut der Universität Zürich, Switzerland.
- Burmester J
- Plückthun A
- 1996-10-31
Published in:
- Gene. - 1996
Bacterial Proteins
Bacteriophage M13
Base Sequence
Capsid Proteins
Cloning, Molecular
DNA, Recombinant
DNA-Binding Proteins
Escherichia coli Proteins
Gene Expression Regulation
Genetic Vectors
Immunoglobulin Fragments
Lac Repressors
Molecular Sequence Data
Promoter Regions, Genetic
Recombinant Fusion Proteins
Repressor Proteins
Terminator Regions, Genetic
Viral Fusion Proteins
English
Expression of toxic gene products affects bacterial cell growth and phage display, causing a strong selection against plasmid maintenance and integrity. During phage propagation steps, in particular, phagemid instability can dramatically affect diversity of antibody libraries or even lead to the deletion of antibody genes. We constructed a modified phage display vector by introducing a strong transcriptional terminator upstream of the lac promoter, which together with glucose suppression of its CAP-dependent activation, very efficiently represses product formation before induction.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/0378-1119(96)00337-x
- PMID 8921894
- Persistent URL
- https://sonar.ch/global/documents/185619
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