Journal article
Selectively infective phages (SIP).
- Spada S Biochemisches Institut der Universität Zürich, Switzerland.
- Krebber C
- Plückthun A
- 1997-06-01
Published in:
- Biological chemistry. - 1997
Bacteriophages
Evolution, Molecular
Genetic Engineering
Humans
Inoviridae
Peptide Library
Protein Engineering
English
We review here advances in the selectively infective phage (SIP) technology, a novel method for the in vivo selection of interacting protein-ligand pairs. A 'selectively infective phage' consists of two components, a filamentous phage particle made non-infective by replacing its N-terminal domains of gene3 protein (g3p) with a ligand-binding protein, and an 'adapter' molecule in which the ligand is linked to those N-terminal domains of g3p which are missing from the phage particle. Infectivity is restored when the displayed protein binds the ligand and thereby attaches the missing N-terminal domains of g3p to the phage particle. Phage propagation becomes strictly dependent on the protein-ligand interaction. This method shows promise both in the area of library screening and in the optimization of peptides or proteins.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1515/bchm.1997.378.6.445
- PMID 9224923
- Persistent URL
- https://sonar.ch/global/documents/185644
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