Back
Angiotensin II increases vascular and renal endothelin-1 and functional endothelin converting enzyme activity in vivo: role of ETA receptors for endothelin regulation.
Journal article

Angiotensin II increases vascular and renal endothelin-1 and functional endothelin converting enzyme activity in vivo: role of ETA receptors for endothelin regulation.

  • 1997-10-28
Published in:
  • Biochemical and biophysical research communications. - 1997
Angiotensin II
Animals
Aorta
Aspartic Acid Endopeptidases
Blood Pressure
Endothelin Receptor Antagonists
Endothelin-1
Endothelin-Converting Enzymes
Femoral Artery
In Vitro Techniques
Iodine Radioisotopes
Kidney
Male
Metalloendopeptidases
Muscle Contraction
Phenylpropionates
Pyrimidines
Rats
Rats, Inbred WKY
Receptor, Endothelin A
Receptors, Endothelin
English Angiotensin II (Ang II)-stimulated expression of endothelin-1 (ET-1) mRNA is blocked by ETA antagonists in vitro. We studied effects of Ang II (200 ng/kg/min) and ETA antagonist LU135252 (50 mg/kg/d) in WKY rats in vivo investigating vascular and renal ET-1 protein expression, functional endothelin converting enzyme (ECE) activity, and clearance of (125I)ET-1. Infusion of Ang II for two weeks increased ET-1 protein content in aorta (4.7-fold) and femoral artery (1.6-fold) with and without endothelium and in kidneys (3-fold, p<0.05) and enhanced functional ECE activity (p<0.05). The Ang II-induced increase in tissue ET-1 content and functional ECE activity was completely prevented by LU135252 (p<0.05). Chronic treatment of control animals with LU135252 lowered basal vascular but not renal ET-1 content (p<0.05 vs. control). Clearance of 125IET-1 was unaffected by the treatments. It is concluded that Ang II increases ET-1 protein and functional ECE activity in vascular smooth muscle and kidney through ETA-receptors in vivo.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/185654
Statistics
Document views: 22 File downloads: