Extracerebral Tissue Damage in the Intraluminal Filament Mouse Model of Middle Cerebral Artery Occlusion.

Vaas M; Ni R; Rudin M; Kipar A; Klohs J

doi:10.3389/fneur.2017.00085

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Journal article

Extracerebral Tissue Damage in the Intraluminal Filament Mouse Model of Middle Cerebral Artery Occlusion.

Vaas M Institute for Biomedical Engineering, University of Zurich, ETH , Zurich , Switzerland.
Ni R Institute for Biomedical Engineering, University of Zurich, ETH , Zurich , Switzerland.
Rudin M Institute for Biomedical Engineering, University of Zurich, ETH, Zurich, Switzerland; Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.
Kipar A Institute of Veterinary Pathology, University of Zurich , Zurich , Switzerland.
Klohs J Institute for Biomedical Engineering, University of Zurich, ETH , Zurich , Switzerland.

2017-03-29

Published in:

Frontiers in neurology. - 2017

middle cerebral artery occlusion

English Middle cerebral artery occlusion is the most common model of focal cerebral ischemia in the mouse. In the surgical procedure, the external carotid artery (ECA) is ligated; however, its effect on the tissue supplied by the vessel has not been described so far. C57BL/6 mice underwent 1 h of transient MCAO (tMCAO) or sham surgery. Multi-spectral optoacoustic tomography was employed at 30 min after surgery to assess oxygenation in the temporal muscles. Microstructural changes were assessed with magnetic resonance imaging and histological examination at 24 h and 48 h after surgery. Ligation of the ECA resulted in decreased oxygenation of the left temporal muscle in most sham-operated and tMCAO animals. Susceptible mice of both groups exhibited increased T2 relaxation times in the affected muscle with histological evidence of myofibre degeneration, interstitial edema, and neutrophil influx. Ligatures had induced an extensive neutrophil-dominated inflammatory response. ECA ligation leads to distinct hypoxic degenerative changes in the tissue of the ECA territory and to ligature-induced inflammatory processes. An impact on outcome needs to be considered in this stroke model.

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