Characterization of Mouse Mesenteric Lymphatic Valve Structure and Function.
- Sabine A Department of Oncology, Division of Experimental Pathology, CHUV, Ludwig Institute for Cancer Research, University of Lausanne, Epalinges, Switzerland. amelie.sabine@unil.ch.
- Davis MJ Medical Pharmacology & Physiology, University of Missouri, Columbia, MO, USA.
- Bovay E Department of Oncology, Division of Experimental Pathology, CHUV, Ludwig Institute for Cancer Research, University of Lausanne, Epalinges, Switzerland.
- Petrova TV Department of Oncology, Division of Experimental Pathology, CHUV, Ludwig Institute for Cancer Research, University of Lausanne, Epalinges, Switzerland. tatiana.petrova@unil.ch.
- 2018-09-23
Published in:
- Methods in molecular biology (Clifton, N.J.). - 2018
Collecting vessel
Lymphatic valve
Mesentery
Angiography
Animals
Fluorescent Antibody Technique
Image Processing, Computer-Assisted
Lymphatic Vessels
Mesentery
Mice
Microscopy, Fluorescence
English
Intraluminal valves of collecting lymphatic vessels ensure unidirectional lymph transport against hydrostatic pressure gradient. Mouse mesentery harbors up to 800 valves and represents a convenient model for lymphatic valve quantification, high resolution imaging of different stages of valve development as well as for analysis of valve function. The protocol describes embryonic and postnatal mesenteric lymphatic vessel preparation for whole-mount immunofluorescent staining and visualization of valve organization, quantification of main morphological parameters such as valve size and leaflet length, and the quantitative assessment of functional properties of adult valves using back-leak and closure tests.
- Language
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- English
- Open access status
- green
- Identifiers
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- DOI 10.1007/978-1-4939-8712-2_7
- PMID 30242755
- Persistent URL
- https://sonar.ch/global/documents/186170
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