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Biosynthesis of active spinach-chloroplast thioredoxin f in transformed E. coli.
Journal article

Biosynthesis of active spinach-chloroplast thioredoxin f in transformed E. coli.

  • 1992-10-01
Published in:
  • Plant molecular biology. - 1992
Amino Acid Sequence
Base Sequence
Chloroplast Thioredoxins
Chloroplasts
Cloning, Molecular
DNA
Escherichia coli
Immunoblotting
Isoelectric Focusing
Molecular Sequence Data
Plant Proteins
Plants
Recombinant Proteins
Thioredoxins
Transformation, Bacterial
English The recently cloned gene for spinach chloroplast thioredoxin f was subcloned in a modified pKK233-2 expression vector and used for transformation of Escherichia coli cells containing the Iq plasmid. After induction with IPTG (isopropyl-beta-D-thiogalactoside) the transformed cells produce the chloroplast protein in large amounts as insoluble deposit within the cell. The protein has been solubilized, purified and analysed for activity. It shows no difference in catalytic activity from native spinach chloroplast thioredoxin f. Its electrophoretic behaviour suggests that the native thioredoxin f may have a different N-terminus than was assumed on the basis of the protein sequencing results.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/186329
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