First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey.

Lara E; Fernández LD; Schiaffino MR; Izaguirre I

doi:10.1016/j.ejop.2017.05.008

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First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey.

Lara E Laboratory of Soil Biology, University of Neuchâtel, Rue Emile Argand 11, Neuchâtel CH-2000, Switzerland; Real Jardín Botánico, CSIC, Plaza de Murillo 2, E-28014 Madrid, Spain. Electronic address: enrique.lara@unine.ch.
Fernández LD Centro de Investigación en Recursos Naturales y Sustentabilidad (CIRENYS), Universidad Bernardo O'Higgins, Fábrica 1990, Santiago, Chile.
Schiaffino MR Centro de Investigaciones y transferencia del Noroeste de la Provincia de Buenos Aires (CITNOBA, CONICET-UNNOBA), 6000 Junín, Argentina.
Izaguirre I Departamento de Ecología, Genética y Evolución, IEGEBA (UBA-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.

2017-06-30

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European journal of protistology. - 2017

English We characterized molecularly the first freshwater member ever reported for the family Bathycoccaceae in Lake Musters (Argentinean Patagonia). Members of this family are extremely numerous and play a key ecological role in marine systems as primary producers. We cloned a fragment comprising the SSU rRNA gene+ITS region from environmental DNA using specific mamiellophyte primers. The unique SSU rRNA gene sequence obtained clustered robustly with Bathycoccus prasinos. Analysis of the two-dimensional structure of the ITS region showed the presence of a typical supplementary helix in the ITS-2 region, a synapomorphy of Bathycoccaceae, which confirmed further its phylogenetic placement. We finally discuss the possible causes for the presence of this organism in Lake Musters.

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