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First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey.
Journal article

First freshwater member ever reported for the family Bathycoccaceae (Chlorophyta; Archaeplastida) from Argentinean Patagonia revealed by environmental DNA survey.

  • Lara E Laboratory of Soil Biology, University of Neuchâtel, Rue Emile Argand 11, Neuchâtel CH-2000, Switzerland; Real Jardín Botánico, CSIC, Plaza de Murillo 2, E-28014 Madrid, Spain. Electronic address: enrique.lara@unine.ch.
  • Fernández LD Centro de Investigación en Recursos Naturales y Sustentabilidad (CIRENYS), Universidad Bernardo O'Higgins, Fábrica 1990, Santiago, Chile.
  • Schiaffino MR Centro de Investigaciones y transferencia del Noroeste de la Provincia de Buenos Aires (CITNOBA, CONICET-UNNOBA), 6000 Junín, Argentina.
  • Izaguirre I Departamento de Ecología, Genética y Evolución, IEGEBA (UBA-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • 2017-06-30
Published in:
  • European journal of protistology. - 2017
Mamiellophyceae
Picoplankton
Ribosomal genes
Southern Patagonia
Chlorophyta
DNA, Ribosomal
DNA, Ribosomal Spacer
Lakes
Metagenomics
Phylogeny
Species Specificity
English We characterized molecularly the first freshwater member ever reported for the family Bathycoccaceae in Lake Musters (Argentinean Patagonia). Members of this family are extremely numerous and play a key ecological role in marine systems as primary producers. We cloned a fragment comprising the SSU rRNA gene+ITS region from environmental DNA using specific mamiellophyte primers. The unique SSU rRNA gene sequence obtained clustered robustly with Bathycoccus prasinos. Analysis of the two-dimensional structure of the ITS region showed the presence of a typical supplementary helix in the ITS-2 region, a synapomorphy of Bathycoccaceae, which confirmed further its phylogenetic placement. We finally discuss the possible causes for the presence of this organism in Lake Musters.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/186446
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