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Covalent attachment of hybridizable oligonucleotides to glass supports.
Journal article

Covalent attachment of hybridizable oligonucleotides to glass supports.

  • Joos B Department of Medicine, University Hospital, Zurich, Switzerland. infjob@usz.unizh.ch
  • Kuster H
  • Cone R
  • 1997-04-05
Published in:
  • Analytical biochemistry. - 1997
Base Sequence
Binding Sites
Glass
HIV-1
Molecular Structure
Nucleic Acid Hybridization
Oligodeoxyribonucleotides
Oligonucleotide Probes
Oligonucleotides
Silanes
English A simple, rapid, and efficient method for the covalent binding of oligonucleotides to solid glass supports was developed. Glass slides were derivatized with aminophenyl or aminopropyl silanes and 5'-succinylated target oligonucleotides were attached by carbodiimide-mediated coupling. Approximately 40 to 50% of the applied target oligonucleotides covalently bound to the derivatized glass. Hybridizations with radioactively labeled oligonucleotide probes showed that up to 90% of the attached oligonucleotides were available for hybridization. This system can conveniently be applied for studies on hybridization and detection of nucleic acids.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/188481
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