Journal article
An Intrasplenic Injection Model for the Study of Cancer Stem Cell Seeding Capacity.
- Dafflon C Disease Area Oncology, Novartis Institutes for BioMedical Research, Basel, Switzerland.
- Santamaría-Martínez A Tumor Ecology Lab, Department of Oncology, Microbiology and Immunology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland.
- Ordóñez-Morán P Division of Cancer & Stem Cells, School of Medicine, Biodiscovery Institute, Centre for Cancer Sciences, University of Nottingham, Nottingham, UK. Paloma.ordonezmoran@nottingham.ac.uk.
- 2020-07-25
Published in:
- Methods in molecular biology (Clifton, N.J.). - 2020
English
In many tumor types, only a minor pool of cancer cells-the so-called cancer stem cells-is able to colonize distant organs and give rise to secondary tumors. In humans, the liver is one of the main target organs for many metastatic tumor types, including colorectal cancer. However, mouse tumour models only rarely spontaneously metastasize to the liver. Therefore, reliable in vivo experimental metastasis assays are crucial to study cell seeding capacity and the mechanisms controlling these metastatic stem cell properties. Here, we describe an intrasplenic injection model that mimics the process of liver metastasis occurring in cancer patients.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1007/978-1-0716-0747-3_20
- PMID 32705651
- Persistent URL
- https://sonar.ch/global/documents/189383
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