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Label-Free Biosensor Detection of Endocrine Disrupting Compounds Using Engineered Estrogen Receptors.

  • La Spina R European Commission-DG Joint Research Centre, Directorate Health Consumer and Reference Materials, 21027 Ispra, Italy. Rita.LA-SPINA@ec.europa.eu.
  • Ferrero VEV European Commission-DG Joint Research Centre, Directorate Sustainable Resources, 21027 Ispra, Italy. valentina.ferrero81@gmail.com.
  • Aiello V European Commission-DG Joint Research Centre, Directorate Health Consumer and Reference Materials, 21027 Ispra, Italy. vaiello79@gmail.com.
  • Pedotti M Institute for Research in Biomedicine, Università della Svizzera italiana (USI), 6500 Bellinzona, Switzerland. mattia.pedotti@irb.usi.ch.
  • Varani L Institute for Research in Biomedicine, Università della Svizzera italiana (USI), 6500 Bellinzona, Switzerland. luca.varani@irb.usi.ch.
  • Lettieri T European Commission-DG Joint Research Centre, Directorate Sustainable Resources, 21027 Ispra, Italy. teresa.lettieri@ec.europa.eu.
  • Calzolai L European Commission-DG Joint Research Centre, Directorate Health Consumer and Reference Materials, 21027 Ispra, Italy. luigi.calzolai@ec.europa.eu.
  • Haasnoot W Authenticity & Bioassays, RIKILT Wageningen University & Research, Wageningen University, 6708 WB Wageningen, The Netherlands. willem.haasnoot@wur.nl.
  • Colpo P European Commission-DG Joint Research Centre, Directorate Health Consumer and Reference Materials, 21027 Ispra, Italy. pascal.colpo@ec.europa.eu.
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  • 2017-12-23
Published in:
  • Biosensors. - 2017
17β-estradiol
amplification
estrogen receptor
label-free assay
surface plasmon resonance
water
Endocrine Disruptors
Peptides
Protein Engineering
Receptors, Estrogen
Surface Plasmon Resonance
English Endocrine Disrupting Compounds (EDCs) are chemical substances shown to interfere with endogenous hormones affecting the endocrine, immune and nervous systems of mammals. EDCs are the causative agents of diseases including reproductive disorders and cancers. This highlights the urgency to develop fast and sensitive methods to detect EDCs, which are detrimental even at very low concentrations. In this work, we propose a label-free surface plasmon resonance (SPR) biosensor method to detect specific EDCs (17 β-estradiol (E2), ethinyl-estradiol, 4-nonylphenol, tamoxifen) through their binding to estrogen receptor alpha (ERα). We show that the use of rationally designed ERα (as bio-recognition element) in combination with conformation-sensitive peptides (as amplification agent, resulting in increased responses) enables the detection of low parts per billion (ppb) levels of E2. As a proof of concept, this bioassay was used to detect E2 in (spiked) real water samples from fish farms, rivers and the sea at low ppb levels after concentration by solid phase extraction. In addition, the present SPR assay that combines a conformation-sensitive peptide with an array of ERα mutants is very promising for the assessment of the risk of potential estrogenic activity for chemical substances.
Language
  • English
Open access status
gold
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Persistent URL
https://sonar.ch/global/documents/189932
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