Back
Full-text
Journal article

Measurement of long-chain fatty acid uptake into adipocytes.

  • Dubikovskaya E Department of Chemistry, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Chudnovskiy R Department of Nutritional Science and Toxicology, UC Berkeley, Berkeley, California, USA.
  • Karateev G Department of Chemistry, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Park HM Department of Nutritional Science and Toxicology, UC Berkeley, Berkeley, California, USA.
  • Stahl A Department of Nutritional Science and Toxicology, UC Berkeley, Berkeley, California, USA. Electronic address: astahl@berkeley.edu.
  • 2014-02-18
Published in:
  • Methods in enzymology. - 2014
Adipocytes
Brown adipose tissue
Fatty acid transport proteins
Fatty acid uptake
In vivo imaging
Long-chain fatty acids
White adipose tissue
3T3-L1 Cells
Adipocytes
Adipose Tissue
Animals
Biological Transport
Boron Compounds
Cells, Cultured
Fatty Acids
Fluorescent Dyes
Humans
Luminescent Agents
Luminescent Measurements
Mice
Molecular Probes
Optical Imaging
Whole Body Imaging
English The ability of white and brown adipose tissue to efficiently take up long-chain fatty acids is key to their physiological functions in energy storage and thermogenesis, respectively. Several approaches have been taken to determine uptake rates by cultured cells and primary adipocytes including radio- and fluorescently labeled fatty acids. In addition, the recent description of activatable bioluminescent fatty acids has opened the possibility for expanding these in vitro approaches to real-time monitoring of fatty acid uptake kinetics by adipose depots in vivo. Here, we will describe some of the most useful experimental paradigms to quantitatively determine long-chain fatty acid uptake by adipocytes in vitro and provide the reader with detailed instruction on how bioluminescent probes for in vivo imaging can be synthesized and used in living mice.
Language
  • English
Open access status
green
Identifiers
Persistent URL
https://sonar.ch/global/documents/190364
Statistics
Document views: 24 File downloads:
  • Full-text: 0