Fluorogenic probes for live-cell imaging of the cytoskeleton.
Journal article

Fluorogenic probes for live-cell imaging of the cytoskeleton.

  • Lukinavičius G 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2].
  • Reymond L 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland. [3].
  • D'Este E Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
  • Masharina A Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Göttfert F Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
  • Ta H Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
  • Güther A Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University, Jena, Germany.
  • Fournier M Bioimaging and Optics Platform, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Rizzo S Max Planck Institute of Molecular Physiology, Dortmund, Germany.
  • Waldmann H Max Planck Institute of Molecular Physiology, Dortmund, Germany.
  • Blaukopf C Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.
  • Sommer C Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.
  • Gerlich DW Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.
  • Arndt HD Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University, Jena, Germany.
  • Hell SW Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
  • Johnsson K 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland.
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  • 2014-05-27
Published in:
  • Nature methods. - 2014
English We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.
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  • English
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https://sonar.ch/global/documents/190385
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