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Membrane water for probing neuronal membrane potentials and ionic fluxes at the single cell level.

  • Didier MEP Laboratory for fundamental BioPhotonics (LBP), Institute of Bioengineering (IBI), and Institute of Materials Science (IMX), School of Engineering (STI), and Lausanne Centre for Ultrafast Science (LACUS), École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
  • Tarun OB Laboratory for fundamental BioPhotonics (LBP), Institute of Bioengineering (IBI), and Institute of Materials Science (IMX), School of Engineering (STI), and Lausanne Centre for Ultrafast Science (LACUS), École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
  • Jourdain P Laboratory of Neuroenergetics and cellular dynamics, Brain Mind Institute, École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
  • Magistretti P Laboratory of Neuroenergetics and cellular dynamics, Brain Mind Institute, École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
  • Roke S Laboratory for fundamental BioPhotonics (LBP), Institute of Bioengineering (IBI), and Institute of Materials Science (IMX), School of Engineering (STI), and Lausanne Centre for Ultrafast Science (LACUS), École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland. sylvie.roke@epfl.ch.
  • 2018-12-13
Published in:
  • Nature communications. - 2018
Animals
Cell Membrane
Ions
Kinetics
Membrane Potentials
Mice
Neurons
Patch-Clamp Techniques
Single-Cell Analysis
Water
English Neurons communicate through electrochemical signaling within a complex network. These signals are composed of changes in membrane potentials and are traditionally measured with the aid of (toxic) fluorescent labels or invasive electrical probes. Here, we demonstrate an improvement in label-free second harmonic neuroimaging sensitivity by ~3 orders of magnitude using a wide-field medium repetition rate illumination. We perform a side-by-side patch-clamp and second harmonic imaging comparison to demonstrate the theoretically predicted linear correlation between whole neuron membrane potential changes and the square root of the second harmonic intensity. We assign the ion induced changes to the second harmonic intensity to changes in the orientation of membrane interfacial water, which is used to image spatiotemporal changes in the membrane potential and K+ ion flux. We observe a non-uniform spatial distribution and temporal activity of ion channels in mouse brain neurons.
Language
  • English
Open access status
gold
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Persistent URL
https://sonar.ch/global/documents/190733
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