Modulation of nuclear pore topology by transport modifiers.
- Jäggi RD Solid State Physics Laboratory, ETH Zürich, Switzerland.
- Franco-Obregón A
- Mühlhäusser P
- Thomas F
- Kutay U
- Ensslin K
- 2003-01-14
Published in:
- Biophysical journal. - 2003
Active Transport, Cell Nucleus
Alcohols
Amino Acids
Animals
Cell Nucleus
Cells, Cultured
Microscopy, Atomic Force
Nuclear Pore
Oocytes
Surface-Active Agents
Xenopus
beta Karyopherins
English
The nuclear pore complex (NPC) represents the only pathway for macromolecular communication between the nuclear and cytoplasmic compartments of the cell. Nucleocytoplasmic transport requires the interaction of transport receptors with phenylalanine-glycine (FG)-repeats that line the transport pathway through the NPC. Here we examine the effects of transport receptors and amphipathic alcohols on NPC topology using scanning force microscopy. We show that transport receptors that irreversibly bind FG-repeats increase NPC vertical aspect, whereas transport receptors that weakly interact with FG-repeats increase NPC diameter. Interestingly, small polar alcohols likewise increase NPC diameter. These opposing effects agree with the inhibition or enhancement of nuclear transport, respectively, previously ascribed to these agents.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1016/S0006-3495(03)74886-3
- PMID 12524319
- Persistent URL
- https://sonar.ch/global/documents/190908
Statistics
Document views: 22
File downloads:
- Full-text: 0