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The ectomycorrhizal morphotype Pinirhiza sclerotia is formed by Acephala macrosclerotiorum sp. nov., a close relative of Phialocephala fortinii.
Journal article

The ectomycorrhizal morphotype Pinirhiza sclerotia is formed by Acephala macrosclerotiorum sp. nov., a close relative of Phialocephala fortinii.

  • Münzenberger B Institute of Landscape Matter Dynamics, Leibniz-Centre for Agricultural Landscape Research (ZALF), Eberswalder Straße 84, 15374, Müncheberg, Germany. bmuenzenberger@zalf.de.
  • Bubner B Institute of Landscape Matter Dynamics, Leibniz-Centre for Agricultural Landscape Research (ZALF), Eberswalder Straße 84, 15374, Müncheberg, Germany.
  • Wöllecke J Brandenburg University of Technology, Chair of Soil Protection and Recultivation, P.O. Box 101344, 03013, Cottbus, Germany.
  • Sieber TN ETH Zürich, Institute of Integrative Biology (IBZ), Forest Pathology and Dendrology, CH-8092, Zürich, Switzerland.
  • Bauer R Eberhard-Karls-University Tübingen, Botanical Institute, Systematic Botany and Mycology, Auf der Morgenstelle 1, 72076, Tübingen, Germany.
  • Fladung M Johann Heinrich von Thünen-Institute (vTI), Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Sieker Landstraße 2, 22927, Grosshansdorf, Germany.
  • Hüttl RF Brandenburg University of Technology, Chair of Soil Protection and Recultivation, P.O. Box 101344, 03013, Cottbus, Germany.
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  • 2009-05-06
Published in:
  • Mycorrhiza. - 2009
Ascomycota
Molecular Sequence Data
Mycelium
Mycorrhizae
Phylogeny
Plant Roots
English Relatively few ectomycorrhizal fungal species are known to form sclerotia. Usually, sclerotia are initiated at the extraradical mycelium. In this study, we present anatomical and ultrastructural evidence for the formation of sclerotia directly in the hyphal mantle of the mycorrhizal morphotype Pinirhiza sclerotia. A dark-pigmented fungal strain was isolated from Pinirhiza sclerotia and identified by molecular tools as Acephala macrosclerotiorum sp. nov., a close relative of Phialocephala fortinii s.l. As dark septate fungi are known to be mostly endophytic, resyntheses with Pinus sylvestris and A. macrosclerotiorum as well as Populus tremula x Populus tremuloides and A. macrosclerotiorum or P. fortinii s.l. were performed under axenic conditions. No mycorrhizas were found when hybrid aspen was inoculated with A. macrosclerotiorum or P. fortinii. However, A. macrosclerotiorum formed true ectomycorrhizas in vitro with P. sylvestris. Anatomical and ultrastructural features of this ectomycorrhiza are presented. The natural and synthesized ectomycorrhizal morphotypes were identical and characterized by a thin hyphal mantle that bore sclerotia in a later ontogenetic stage. The Hartig net was well-developed and grew up to the endodermis. To our knowledge, this is the first evidence at the anatomical and ultrastructural level that a close relative of P. fortinii s.l. forms true ectomycorrhizas with a coniferous host.
Language
  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/191203
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