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Novel methods for biocatalyst screening.
Journal article

Novel methods for biocatalyst screening.

  • Wahler D Departement für Chemie und Biochemie, Universität Bern, Switzerland.
  • Reymond JL
  • 2001-04-03
Published in:
  • Current opinion in chemical biology. - 2001
Carrier Proteins
Catalysis
Chromatography, High Pressure Liquid
Chromogenic Compounds
Combinatorial Chemistry Techniques
Drug Evaluation, Preclinical
Electrophoresis, Capillary
Enzyme-Linked Immunosorbent Assay
Enzymes
Fluorometry
Gene Library
Mass Spectrometry
Selection, Genetic
Substrate Specificity
English There have been a number of recent advances in catalysis assays applicable for screening biocatalyst libraries in high-throughput format. These include instrumental assays such as high-performance liquid chromatography, mass spectrometry, capillary electrophoresis and IR-thermography, reagent-based assays producing spectroscopic signals (UV/VIS or fluorescence) in response to reaction progress, and assays based on fluorogenic or chromogenic substrates. These fluorogenic substrates enable the assaying of a variety of enzymes in enantioselective and stereoselective manner, including alcohol dehydrogenases, aldolases, lipases, amidases, epoxide hydrolases and phosphatases.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/192045
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