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Differential inhibition by alpha- and beta-tocopherol of human erythroleukemia cell adhesion: role of integrins.
Journal article

Differential inhibition by alpha- and beta-tocopherol of human erythroleukemia cell adhesion: role of integrins.

  • Breyer I Institut für Biochemie und Molekularbiologie, Universität Bern, Bern, Switzerland.
  • Azzi A
  • 2001-06-08
Published in:
  • Free radical biology & medicine. - 2001
Antibodies, Monoclonal
Cell Adhesion
Down-Regulation
Flavonoids
Flow Cytometry
Humans
Integrins
Isoenzymes
K562 Cells
Leukemia, Erythroblastic, Acute
MAP Kinase Signaling System
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinases
Naphthalenes
Neoplasm Proteins
Phosphorylation
Protein Kinase C
Protein Kinase C-alpha
Protein Processing, Post-Translational
Tetradecanoylphorbol Acetate
Tumor Cells, Cultured
U937 Cells
Vitamin E
English The effect of alpha- and beta-tocopherol on human erythroleukemia cell (HEL) adhesion induced by phorbol 12-myristate 13-acetate (PMA) has been studied. Adhesion induced by PMA stimulation was prevented by 44.5% by physiological concentrations of alpha-tocopherol. Under the same experimental conditions, beta-tocopherol, an analogue of alpha-tocopherol, produced 11% inhibition of adhesion. Cell response gradually increased from 0 to 24 h of alpha-tocopherol treatment. Only a slight time dependency of beta-tocopherol inhibition was observed. Another human erythroleukemia cell line (K562) and the human monocyte tumor cell line U937 showed 5.0 and 11.2% inhibition, respectively. Similar to alpha-tocopherol, the protein kinase C inhibitor, Calphostin C, and the MAPK inhibitor, PD98059, prevented PMA-induced cell adhesion. An inhibition of ERK-1 phosphorylation was observed for alpha-tocopherol only in HEL, implying that MAP kinase pathway is involved in this cell line. Fluorescence-activated cell sorting (FACS), by using various integrin-specific monoclonal antibodies, has shown that alpha (1-6), beta1, and alphav integrins are less expressed at the cell surface after alpha-tocopherol treatment. Beta-tocopherol treatment was less effective.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/192053
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