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Modern analytical supercritical fluid chromatography using columns packed with sub-2 μm particles: a tutorial.
Journal article

Modern analytical supercritical fluid chromatography using columns packed with sub-2 μm particles: a tutorial.

  • Nováková L Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, Hradec Králové 500 05, Czech Republic. Electronic address: nol@email.cz.
  • Perrenoud AG School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, Geneva 4 1211, Switzerland.
  • Francois I Waters NV/SA, Connexion Business Park, Brusselsesteenweg 500, Zellik B-1731, Belgium.
  • West C University of Orléans, Institute of Organic and Analytical Chemistry (ICOA), CNRS UMR 7311, Pôle de Chimie, BP 6759, Orléans cedex 2 45067, France.
  • Lesellier E University of Orléans, Institute of Organic and Analytical Chemistry (ICOA), CNRS UMR 7311, Pôle de Chimie, BP 6759, Orléans cedex 2 45067, France.
  • Guillarme D School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Boulevard d'Yvoy 20, Geneva 4 1211, Switzerland.
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  • 2014-04-25
Published in:
  • Analytica chimica acta. - 2014
Column selection
Mobile phase selection
Sub-2μm particles
Supercritical fluid chromatography
Tutorial
Ultra-high performance supercritical fluid chromatography
Chromatography, Supercritical Fluid
Particle Size
Solvents
English This tutorial provides an overview of the possibilities, limitations and analytical conditions of modern analytical supercritical fluid chromatography (SFC) using columns packed with sub-2 μm particles. In particular, it gives a detailed overview of commercially available modern SFC instrumentation and the detectors that can be employed (UV, MS, ELSD, FID, etc.). Some advice on the choice of the stationary phase dimensions and chemistries, the nature of the mobile phase (choice of organic modifier and additives) and its flow rate as well as the backpressure and temperature are also provided. Finally, several groups of potentially problematic compounds, including lipophilic compounds, hydrophilic substances and basic drugs, are discussed in detail. All these families of analytes can be resolved with SFC but require specific analytical conditions.
Language
  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/192111
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