Journal article
Eosinophil extracellular DNA traps in skin diseases.
- Simon D Department of Dermatology, Inselspital, Bern University Hospital, Bern, Switzerland. dagmar.simon@insel.ch
- Hoesli S
- Roth N
- Staedler S
- Yousefi S
- Simon HU
- 2011-01-08
Published in:
- The Journal of allergy and clinical immunology. - 2011
DNA
Eosinophil Granule Proteins
Eosinophils
Extracellular Matrix
Fluorescent Antibody Technique
Humans
Microscopy, Confocal
Skin Diseases
English
BACKGROUND
In the skin, eosinophils are found in a broad spectrum of diseases, including infectious diseases.
OBJECTIVE
In this study, we investigated whether eosinophil extracellular traps, structures containing DNA in association with eosinophil granule proteins able to bind and kill bacteria, are present in the skin under various pathologic conditions.
METHODS
Immunofluorescence staining was performed on sections of paraformaldehyde-fixed and paraffin-embedded skin biopsy tissues of 25 different eosinophilic skin diseases by using propidium iodide and an antibody to eosinophil cationic protein. Slides were evaluated by laser scanning microscopy.
RESULTS
Eosinophils releasing DNA together with eosinophil cationic protein were detected in infectious skin diseases such as ectoparasitosis and larva migrans. Further, we observed the extracellular DNA structures in allergic/reactive diseases (Wells syndrome, hypereosinophilic syndrome, positive reaction of atopy patch test, allergic contact dermatitis, drug hypersensitivity) and in autoimmune diseases (bullous pemphigoid, pemphigus foliaceus, dermatitis herpetiformis). The average number of eosinophils releasing DNA in the skin was usually below 10%, although in Wells syndrome the proportion was up to 30%. In areas with clusters of eosinophils, up to 50% of the eosinophils were seen to generate eosinophil extracellular traps.
CONCLUSION
Eosinophil extracellular traps are seen in both infectious and noninfectious inflammatory skin diseases and are particularly common in Wells syndrome.
In the skin, eosinophils are found in a broad spectrum of diseases, including infectious diseases.
OBJECTIVE
In this study, we investigated whether eosinophil extracellular traps, structures containing DNA in association with eosinophil granule proteins able to bind and kill bacteria, are present in the skin under various pathologic conditions.
METHODS
Immunofluorescence staining was performed on sections of paraformaldehyde-fixed and paraffin-embedded skin biopsy tissues of 25 different eosinophilic skin diseases by using propidium iodide and an antibody to eosinophil cationic protein. Slides were evaluated by laser scanning microscopy.
RESULTS
Eosinophils releasing DNA together with eosinophil cationic protein were detected in infectious skin diseases such as ectoparasitosis and larva migrans. Further, we observed the extracellular DNA structures in allergic/reactive diseases (Wells syndrome, hypereosinophilic syndrome, positive reaction of atopy patch test, allergic contact dermatitis, drug hypersensitivity) and in autoimmune diseases (bullous pemphigoid, pemphigus foliaceus, dermatitis herpetiformis). The average number of eosinophils releasing DNA in the skin was usually below 10%, although in Wells syndrome the proportion was up to 30%. In areas with clusters of eosinophils, up to 50% of the eosinophils were seen to generate eosinophil extracellular traps.
CONCLUSION
Eosinophil extracellular traps are seen in both infectious and noninfectious inflammatory skin diseases and are particularly common in Wells syndrome.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/j.jaci.2010.11.002
- PMID 21211654
- Persistent URL
- https://sonar.ch/global/documents/192450
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